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Biotin is subsequently generated through the combination of these pathways in a four-step process (148)

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Three of these intermediates, termed pre-B act , B act -I and B act -II, contain the complete NTR complex and three components of the NTC complex (CDC5L, CWC15 and PLRG1), whereas additional NTC components (PRP19, BCAS2) are not yet integrated 16

Blood sampling Rats in the diestrus phase were anesthetized using intraperitoneal injections of Ketamine (50 mg/kg) and Xylazine (5 mg/kg) and blood samples were obtained via cardiac puncture